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Pathoscreen X-Y-Z

Description

Measuring GFP, RFP, CF and colour images of small whole plants, detached leaves and/or pathogens

PathoScreen visualizes the GFP/RFP labelled pathogen and the effect of the presence of the pathogen on the plant through imaging the stress response of the plant. PathoScreen measures multiple fluorescence images at 6 megapixel resolution providing pixel-to-pixel images of Green Fluorescence Protein (GFP),Red Fluorescence Protein (RFP or DsRed), Chlorophyll Fluorescence (CF) and colour (in RGB). Modified pathogens can be selectively imaged using GFP and/or RFP labelling together with the stress response of the plant using chlorophyll fluorescence technology yielding the maximum quantum yield of Photosystem II (PSII) photochemistry (Fv/Fm). This provides early detection of the pathogen and its interaction with the photosynthesis of the plant. GFP images are corrected for auto-fluorescence from senescing leaves.

Technical Details

• Camera with 6 Mp at 14 bit 
• Total recording time of all images in 10 s 
• Time-lapse recording 
• Filtered blue LEDs for GFP excitation, filtered green LEDs for RFP excitation, red LEDs for chlorophyll excitation and white LEDs for colour 
• Pixel-to-pixel information on multi fluorescence and colour images 
• Built-in pc and monitor for timed imaging, light control and data storage 
• Custom optimized measuring protocols
• Fluorescence images for labeled biological material on GFP, auto- fluorescence, and RFP 
• Chlorophyll fluorescence images of plant material yielding F0 and Fm of the Kautsky induction curve 
• Colour images in separate red, green and blue images (RGB) 
• Imaged area of 130×130 mm2 
• High Depth of Field (DOF)
• GFP, GFP corrected (for auto-fluorescence) , RFP and CF 
• Absolute and relative area of pathogens and plant material 
• Fv/FM= (FM-F0)/FM (maximum quantum yield of PSII photochemistry) 
• Colour in red, green and blue intensities (RGB values)

Contact Theo van der Lee
Contact Els Verstappen

Brand

PhenoVation

Organisation

Plant Sciences

Department

Bio Interacties en Plantgezondheid

Last edited by Oscar de Vos on 2017-11-24