The new Sequel System is based on the Single Molecule Real-Time (SMRT) sequencing technology, successfully applied on the Pacbio RS-II system for many years.
SMRT sequencing is built upon two key innovations that overcome major challenges in the field of sequencing. SMRT cells, containing 1 million ZMW wells, allow light to illuminate only at the bottom of hundreds of thousands of well, in which a single DNA polymerase/template complex is immobilized in parallel. Sequence reads are obtained by real time detection of phospho linked fluorescently labelled nucleotides as the DNA polymerase produces a completely natural DNA strand of each individual template molecule.
This technique results in 300,000 to 500,000 reads per SMRT cell, with average read length > 10 Kb. SMRT sequencing is free of systematic errors, resulting in the highest consensus quality of any sequencing technology up to date.